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KMID : 0381120110330030245
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Genes and Genomics
2011 Volume.33 No. 3 p.245 ~ p.245
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Identification of reference genes for normalization of gene expression in thoroughbred and Jeju native horse(Jeju pony) tissues
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Ahn Kung
Bae Jin-Han
Nam Kyu-Hwi
Lee Chong-Eon
Park Kyung-Do
Lee Hak-Kyo
Cho Byung-Wook
Kim Heui-Soo
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Abstract
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Quantitative analysis of horse gene expression profiles under diverse experimental conditions is limited by the lack of reliable reference genes for normalization of mRNA levels. Therefore, in this study, the expression of potential reference genes was compared between thoroughbred and Jeju native horse (Jeju pony). We compared the expression of nine genes by quantitative real-time RT-PCR in fourteen tissues between the two horse breeds and analyzed their stability using the geNorm and NormFinder programs. The data obtained in this study suggest that the UBB gene could serve as a reference gene in gene expression analysis of thoroughbred and Jeju native horses.
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KEYWORD
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Jeju (Cheju) native horse, Normalization, Real-time RT-PCR, Reference genes, Thoroughbred
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